Characterization and high-resolution mapping of a late blight resistance locus similar to R2 in potato.
Identifieur interne : 002218 ( Main/Exploration ); précédent : 002217; suivant : 002219Characterization and high-resolution mapping of a late blight resistance locus similar to R2 in potato.
Auteurs : T H Park [Pays-Bas] ; V G A A. Vleeshouwers ; D J Huigen ; E A G. Van Der Vossen ; H J Van Eck ; R G F. VisserSource :
- TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik [ 0040-5752 ] ; 2005.
Descripteurs français
- KwdFr :
- ADN des plantes (génétique), Cartographie chromosomique (MeSH), Gènes de plante (MeSH), Maladies des plantes (génétique), Maladies des plantes (microbiologie), Phytophthora (pathogénicité), Solanum (génétique), Solanum (microbiologie), Solanum tuberosum (génétique), Solanum tuberosum (microbiologie), Spécificité d'espèce (MeSH).
- MESH :
- génétique : ADN des plantes, Maladies des plantes, Solanum, Solanum tuberosum.
- microbiologie : Maladies des plantes, Solanum, Solanum tuberosum.
- pathogénicité : Phytophthora.
- Cartographie chromosomique, Gènes de plante, Spécificité d'espèce.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : DNA, Plant.
- genetics : Plant Diseases, Solanum, Solanum tuberosum.
- microbiology : Plant Diseases, Solanum, Solanum tuberosum.
- pathogenicity : Phytophthora.
- Chromosome Mapping, Genes, Plant, Species Specificity.
Abstract
Identification of resistance (R) genes to Phytophthora infestans is an essential step in molecular breeding of potato. We identified three specific R genes segregating in a diploid mapping population. One of the R genes is located on chromosome 4 and proved phenotypically indistinguishable from the Solanum demissum-derived R2, although S. demissum is not directly involved in the pedigree of the population. By bulked segregant analysis combined with a resistance assay, a genetic linkage map of the R2-like locus was constructed with 30 coupling and 23 repulsion phase AFLP markers. Two markers flanking the R2-like locus were applied to screen an extended population of 1,586 offspring. About 103 recombinants were selected, and an accurate high-resolution map was constructed. The R2-like resistance was localized in a 0.4 cM interval and was found co-segregating with four AFLP markers, which can be used to isolate the R2-like gene by map-based gene cloning. By analyzing race-specificity and R gene-specific molecular markers, we also found that an R1-like gene and an additional unknown R gene are segregating in the population.
DOI: 10.1007/s00122-005-2050-4
PubMed: 15909136
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Vleeshouwers</name></author><author><name sortKey="Huigen, D J" sort="Huigen, D J" uniqKey="Huigen D" first="D J" last="Huigen">D J Huigen</name></author><author><name sortKey="Van Der Vossen, E A G" sort="Van Der Vossen, E A G" uniqKey="Van Der Vossen E" first="E A G" last="Van Der Vossen">E A G. Van Der Vossen</name></author><author><name sortKey="Van Eck, H J" sort="Van Eck, H J" uniqKey="Van Eck H" first="H J" last="Van Eck">H J Van Eck</name></author><author><name sortKey="Visser, R G F" sort="Visser, R G F" uniqKey="Visser R" first="R G F" last="Visser">R G F. Visser</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15909136</idno><idno type="pmid">15909136</idno><idno type="doi">10.1007/s00122-005-2050-4</idno><idno type="wicri:Area/Main/Corpus">002263</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002263</idno><idno type="wicri:Area/Main/Curation">002263</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002263</idno><idno type="wicri:Area/Main/Exploration">002263</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Characterization and high-resolution mapping of a late blight resistance locus similar to R2 in potato.</title><author><name sortKey="Park, T H" sort="Park, T H" uniqKey="Park T" first="T H" last="Park">T H Park</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant Sciences, Laboratory of Plant Breeding, Graduate School Experimental Plant Sciences, Wageningen University, Binnenhaven 5, 6709 PD, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Plant Sciences, Laboratory of Plant Breeding, Graduate School Experimental Plant Sciences, Wageningen University, Binnenhaven 5, 6709 PD, Wageningen</wicri:regionArea><orgName type="university">Université de Wageningue</orgName><placeName><settlement type="city">Wageningue</settlement><region nuts="2">Gueldre (province)</region></placeName></affiliation></author><author><name sortKey="Vleeshouwers, V G A A" sort="Vleeshouwers, V G A A" uniqKey="Vleeshouwers V" first="V G A A" last="Vleeshouwers">V G A A. Vleeshouwers</name></author><author><name sortKey="Huigen, D J" sort="Huigen, D J" uniqKey="Huigen D" first="D J" last="Huigen">D J Huigen</name></author><author><name sortKey="Van Der Vossen, E A G" sort="Van Der Vossen, E A G" uniqKey="Van Der Vossen E" first="E A G" last="Van Der Vossen">E A G. Van Der Vossen</name></author><author><name sortKey="Van Eck, H J" sort="Van Eck, H J" uniqKey="Van Eck H" first="H J" last="Van Eck">H J Van Eck</name></author><author><name sortKey="Visser, R G F" sort="Visser, R G F" uniqKey="Visser R" first="R G F" last="Visser">R G F. Visser</name></author></analytic><series><title level="j">TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik</title><idno type="ISSN">0040-5752</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Chromosome Mapping (MeSH)</term><term>DNA, Plant (genetics)</term><term>Genes, Plant (MeSH)</term><term>Phytophthora (pathogenicity)</term><term>Plant Diseases (genetics)</term><term>Plant Diseases (microbiology)</term><term>Solanum (genetics)</term><term>Solanum (microbiology)</term><term>Solanum tuberosum (genetics)</term><term>Solanum tuberosum (microbiology)</term><term>Species Specificity (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN des plantes (génétique)</term><term>Cartographie chromosomique (MeSH)</term><term>Gènes de plante (MeSH)</term><term>Maladies des plantes (génétique)</term><term>Maladies des plantes (microbiologie)</term><term>Phytophthora (pathogénicité)</term><term>Solanum (génétique)</term><term>Solanum (microbiologie)</term><term>Solanum tuberosum (génétique)</term><term>Solanum tuberosum (microbiologie)</term><term>Spécificité d'espèce (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Plant</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plant Diseases</term><term>Solanum</term><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN des plantes</term><term>Maladies des plantes</term><term>Solanum</term><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Maladies des plantes</term><term>Solanum</term><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Solanum</term><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Chromosome Mapping</term><term>Genes, Plant</term><term>Species Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cartographie chromosomique</term><term>Gènes de plante</term><term>Spécificité d'espèce</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Identification of resistance (R) genes to Phytophthora infestans is an essential step in molecular breeding of potato. We identified three specific R genes segregating in a diploid mapping population. One of the R genes is located on chromosome 4 and proved phenotypically indistinguishable from the Solanum demissum-derived R2, although S. demissum is not directly involved in the pedigree of the population. By bulked segregant analysis combined with a resistance assay, a genetic linkage map of the R2-like locus was constructed with 30 coupling and 23 repulsion phase AFLP markers. Two markers flanking the R2-like locus were applied to screen an extended population of 1,586 offspring. About 103 recombinants were selected, and an accurate high-resolution map was constructed. The R2-like resistance was localized in a 0.4 cM interval and was found co-segregating with four AFLP markers, which can be used to isolate the R2-like gene by map-based gene cloning. By analyzing race-specificity and R gene-specific molecular markers, we also found that an R1-like gene and an additional unknown R gene are segregating in the population.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15909136</PMID><DateCompleted><Year>2005</Year><Month>10</Month><Day>13</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0040-5752</ISSN><JournalIssue CitedMedium="Print"><Volume>111</Volume><Issue>3</Issue><PubDate><Year>2005</Year><Month>Aug</Month></PubDate></JournalIssue><Title>TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik</Title><ISOAbbreviation>Theor Appl Genet</ISOAbbreviation></Journal><ArticleTitle>Characterization and high-resolution mapping of a late blight resistance locus similar to R2 in potato.</ArticleTitle><Pagination><MedlinePgn>591-7</MedlinePgn></Pagination><Abstract><AbstractText>Identification of resistance (R) genes to Phytophthora infestans is an essential step in molecular breeding of potato. We identified three specific R genes segregating in a diploid mapping population. One of the R genes is located on chromosome 4 and proved phenotypically indistinguishable from the Solanum demissum-derived R2, although S. demissum is not directly involved in the pedigree of the population. By bulked segregant analysis combined with a resistance assay, a genetic linkage map of the R2-like locus was constructed with 30 coupling and 23 repulsion phase AFLP markers. Two markers flanking the R2-like locus were applied to screen an extended population of 1,586 offspring. About 103 recombinants were selected, and an accurate high-resolution map was constructed. The R2-like resistance was localized in a 0.4 cM interval and was found co-segregating with four AFLP markers, which can be used to isolate the R2-like gene by map-based gene cloning. By analyzing race-specificity and R gene-specific molecular markers, we also found that an R1-like gene and an additional unknown R gene are segregating in the population.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Park</LastName><ForeName>T H</ForeName><Initials>TH</Initials><AffiliationInfo><Affiliation>Department of Plant Sciences, Laboratory of Plant Breeding, Graduate School Experimental Plant Sciences, Wageningen University, Binnenhaven 5, 6709 PD, Wageningen, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vleeshouwers</LastName><ForeName>V G A A</ForeName><Initials>VG</Initials></Author><Author ValidYN="Y"><LastName>Huigen</LastName><ForeName>D J</ForeName><Initials>DJ</Initials></Author><Author ValidYN="Y"><LastName>van der Vossen</LastName><ForeName>E A G</ForeName><Initials>EA</Initials></Author><Author ValidYN="Y"><LastName>van Eck</LastName><ForeName>H J</ForeName><Initials>HJ</Initials></Author><Author ValidYN="Y"><LastName>Visser</LastName><ForeName>R G F</ForeName><Initials>RG</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2005</Year><Month>05</Month><Day>21</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Theor Appl Genet</MedlineTA><NlmUniqueID>0145600</NlmUniqueID><ISSNLinking>0040-5752</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018744">DNA, Plant</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002874" MajorTopicYN="N">Chromosome Mapping</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018744" MajorTopicYN="N">DNA, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="Y">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032322" MajorTopicYN="N">Solanum</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011198" MajorTopicYN="N">Solanum tuberosum</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013045" MajorTopicYN="N">Species Specificity</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2005</Year><Month>01</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2005</Year><Month>04</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>5</Month><Day>24</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2005</Year><Month>10</Month><Day>14</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>5</Month><Day>24</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId 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Vossen, E A G" sort="Van Der Vossen, E A G" uniqKey="Van Der Vossen E" first="E A G" last="Van Der Vossen">E A G. Van Der Vossen</name><name sortKey="Van Eck, H J" sort="Van Eck, H J" uniqKey="Van Eck H" first="H J" last="Van Eck">H J Van Eck</name><name sortKey="Visser, R G F" sort="Visser, R G F" uniqKey="Visser R" first="R G F" last="Visser">R G F. Visser</name><name sortKey="Vleeshouwers, V G A A" sort="Vleeshouwers, V G A A" uniqKey="Vleeshouwers V" first="V G A A" last="Vleeshouwers">V G A A. Vleeshouwers</name></noCountry><country name="Pays-Bas"><region name="Gueldre (province)"><name sortKey="Park, T H" sort="Park, T H" uniqKey="Park T" first="T H" last="Park">T H Park</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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